Stains & molecular markers
Cytokeratin AE1 / AE3



Topic Completed: 1 July 2013

Minor changes: 15 September 2020

Copyright: 2003-2020, PathologyOutlines.com, Inc.

PubMed Search: Cytokeratin AE1 / AE3

Nat Pernick, M.D.
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Cite this page: Pernick N. Cytokeratin AE1 / AE3. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsae1ae3.html. Accessed September 23rd, 2020.
Definition / general
  • Keratin cocktail that detects CK1 - 8, 10, 14 - 16 and 19, but does not detect CK17 or CK18
  • Often referred to in literature as "keratin" or "pankeratin", although this may also refer to Cytokeratin MNF116
  • May cross react with GFAP, leading to aberrant staining of glial tumors (ependymoma, glioblastoma, schwannoma, Mod Pathol 2006;19:115)
  • Note: staining pattern of cocktail may be different than staining pattern of AE1 and AE3 separately, due to different pretreatment
Uses by pathologists
Microscopic (histologic) images

Contributed by Andrey Bychkov, M.D., Ph.D.

MALT lymphoma

Strongly positive cancer
cells versus weakly positive
thyroid follicles

Cytology images

Cases #54 and 32

Salivary gland:
epithelial-
myoepithelial
carcinoma

Thyroid anaplastic carcinoma

Kidney: atypical angiomyolipoma -
(left) is negative, normal kidney;
(right) is positive



Images hosted on other servers:

Ascitis fluid: metastatic alveolar
rhabdomyosarcoma is negative,
mesotheilal cell is internal
positive control (fig C)

Breast lobular carcinoma: metastasis to lymph node - H&E and AE1 / AE3


Duodenum: neuroendocrine carcinoma

Liver: adenomatoid tumor (fig 3)

Lung cancer
(non small cell)
associated
lymph node

Lung cancer (non small cell) associated lymph node: frozen section


Oral cavity:
squamous cell
carcinoma -
acantholytic type

Pleura: sarcomatoid mesothelioma (fig A)

Soft tissue: benign mixed tumor (fig B)

Soft tissue: myoepithelioma (fig C in both images)


Soft tissue: myxoid chondrosarcoma (fig B)

Spinal cord:
myxopapillary
ependymoma of
filum terminale

Testis: yolk sac tumor

Positive staining - normal
Positive staining - disease
Negative stains
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Image 01 Image 02