Stains
Cytokeratin AE1 / AE3

Author: Nat Pernick, M.D. (see Authors page)

Revised: 2 December 2016, last major update July 2013

Copyright: (c) 2003-2016, PathologyOutlines.com, Inc.

PubMed Search: Cytokeratin AE1 / AE3
Cite this page: Cytokeratin AE1 / AE3. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsae1ae3.html. Accessed December 4th, 2016.
Definition / General
  • Keratin cocktail that detects CK1 - 8, 10, 14 - 16 and 19, but does not detect CK17 or CK18
  • Often referred to in literature as "keratin" or "pankeratin", although this may also refer to Cytokeratin MNF116
  • May cross react with GFAP, leading to aberrant staining of glial tumors (ependymoma, glioblastoma, schwannoma, Mod Pathol 2006;19:115)
  • Note: staining pattern of cocktail may be different than staining pattern of AE1 and AE3 separately, due to different pretreatment
Uses by Pathologists
Micro Images
Images hosted on PathOut server:

Courtesy of Andrey Bychkov, M.D., Ph.D.

Strongly positive cancer cells vs.
weakly positive thyroid follicles

Cytology Images
Scroll to see all images.

Images hosted on PathOut server:

Salivary gland:
epithelial-
myoepithelial
carcinoma

Thyroid anaplastic carcinoma

Kidney: atypical angiomyolipoma -
(left) is negative, normal kidney;
(right) is positive



Images hosted on other servers:

Ascitis fluid: metastatic alveolar
rhabdomyosarcoma is negative,
mesotheilal cell is internal
positive control (fig C)


Breast ductal carcinoma: nodal metastases

Breast lobular carcinoma: metastasis to lymph node - H&E and AE1 / AE3

Breast:
angiomyoepithelioma -
malignant, epithelial
component (fig C)


Duodenum: neuroendocrine carcinoma

Liver: adenomatoid tumor (fig 3)

Lung cancer
(non small cell)
associated
lymph node

Lung cancer (non small cell) associated lymph node: frozen section

Nasal cavity:
small cell
carcinoma
(fig 1D)

Oral cavity:
squamous cell
carcinoma -
acantholytic type

Pleura: poorly differentiated carcinoma

Pleura: sarcomatoid mesothelioma (fig A)

Salivary gland: acinic cell carcinoma (left, fig 4); undifferentiated sarcoma - normal ducts (right, fig b)

Skin: basal cell
carcinoma
metastatic
to bone (fig 4)

Small bowel:
sarcomatoid
carcinoma
(fig 3A)

Soft tissue: angiosarcoma - left, fig 2; right, fig 1C

Soft tissue: benign mixed tumor (fig B)

Soft tissue: myoepithelioma (fig C in both images)


Soft tissue: myxoid chondrosarcoma (fig B)

Spinal cord:
myxopapillary
ependymoma of
filum terminale

Testis: yolk sac tumor

Testis: adenomatoid tumor



Images from Nature.com:

Adrenocortical carcinoma: oncocytic

Retroperitoneum: schwannoma

Vagina: mixed tumor (fig B)


Positive Staining - Normal
Positive Staining - Disease
Negative Stains