Stains & CD markers
Cytokeratin AE1 / AE3

Last author update: 1 July 2013
Last staff update: 3 May 2022

Copyright: 2003-2022,, Inc.

PubMed Search: Cytokeratin AE1 / AE3

Nat Pernick, M.D.
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Cite this page: Pernick N. Cytokeratin AE1 / AE3. website. Accessed May 18th, 2022.
Definition / general
  • Keratin cocktail that detects CK1 - 8, 10, 14 - 16 and 19, but does not detect CK17 or CK18
  • Often referred to in literature as "keratin" or "pankeratin", although this may also refer to Cytokeratin MNF116
  • May cross react with GFAP, leading to aberrant staining of glial tumors (ependymoma, glioblastoma, schwannoma, Mod Pathol 2006;19:115)
  • Note: staining pattern of cocktail may be different than staining pattern of AE1 and AE3 separately, due to different pretreatment
Uses by pathologists
Microscopic (histologic) images

Contributed by Andrey Bychkov, M.D., Ph.D.

MALT lymphoma

Strongly positive cancer
cells versus weakly positive
thyroid follicles

Cytology images

Cases #54 and 32

Salivary gland:

Thyroid anaplastic carcinoma

Kidney: atypical

Images hosted on other servers:

Various stains

Breast lobular carcinoma: metastasis to lymph node - H&E and AE1 / AE3

Duodenum: neuroendocrine carcinoma

Liver: adenomatoid tumor

Lung cancer
(non small cell)
lymph node

Lung cancer (non small cell) associated lymph node: frozen section

Oral cavity:
squamous cell
carcinoma -
acantholytic type

Pleura: sarcomatoid mesothelioma

Soft tissue: benign mixed tumor

Soft tissue: myoepithelioma

Soft tissue: myxoid chondrosarcoma

Spinal cord:
ependymoma of
filum terminale

Testis: yolk sac tumor

Positive staining - normal
Positive staining - disease
Negative stains
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