Stains
Cyclin D1 / bcl1

Author: Nat Pernick, M.D. (see Authors page)

Revised: 30 October 2015, last major update March 2013

Copyright: (c) 2003-2015, PathologyOutlines.com, Inc.

PubMed Search: Cyclin+D1[title]
Cite this page: Cyclin D1 . PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainscyclind1.html. Accessed August 20th, 2017.
Definition / general
  • Also called bcl1 - "b cell lymphoma #1"
  • Coded by BCL1 / PRAD1 (parathyroid adenoma 1) gene on #11q13
  • Responsible for transition to S phase by phosphorylating the retinoblastoma gene product, which releases transcription factors to initiate DNA replication
  • Overexpression promotes transformation to a malignant phenotype; overexpressed in many tumors
  • Cyclin D1 genotypes may be associated with nasopharyngeal carcinoma risk (Anticancer Res 2012;32:1093)
Uses by pathologists
  • Interpretation: nuclear stain
  • Mantle cell lymphoma: characterized by t(11;14) translocation, which places the cyclin D1 gene next to an immunoglobulin heavy chain enhancer gene; among lymphomas, cyclin D1 expression is very specific for mantle cell lymphoma; however, blastoid cases may be negative (Am J Surg Pathol 2012;36:214)
  • Parathyroid: overexpressed in 40% of parathyroid adenomas, strong staining in carcinomas
Microscopic (histologic) images

Images hosted on Pathout server:

Breast: MALT lymphoma is cyclin D1 negative



Images hosted on other servers:

Breast: papilloma and papillary carcinoma

Cervix: various benign and malignant lesions

Mantle cell lympoma (small bowel)


Mantle cell lympoma (axillary lymph node)

Skin: normal

Skin: compound Spitz nevus and melanoma


Stomach: gastric or gastroesophageal junction adenocarcinoma

Uterus: normal, complex hyperplasia and carcinoma

Various images

Virtual slides

Breast: papilloma and papillary carcinoma

Molecular / cytogenetics images

Images hosted on Nature.com:

FISH in mantle cell lymphoma - blastoid variant

FISH in mantle cell lymphoma: cyclin D1/IgH single and double-fusion-positive cells in peripheral
blood sample showing mantle cell lymphoma involvement

Positive staining - normal
Positive staining - disease
Board review question #1
A t(11;14) translocation that creates a CyclinD1-IgH fusion gene is associated with which of the malignancies below?

  1. Burkitt lymphoma
  2. Extranodal marginal zone lymphoma
  3. Follicular lymphoma
  4. Mantle cell lymphoma
  5. Multiple myeloma
Board review answer #1
D. Mantle cell lymphoma.

  1. Incorrect: Burkitt lymphomas are associated with translocations involving the c-MYC gene on chromosome 8.
  2. Incorrect: extranodal marginal zone lymphomas are associated with the following translocations: t(11;18) (creating a MALT1-IAP2 fusion gene), t(1;14)(creating a BCL10-IgH fusion gene) and t(14;18) (creating a MALT1-IgH fusion gene).
  3. Incorrect: follicular lymphoma is associated with a t(14;18) translocation that creates a BCL2-IgH fusion gene.
  1. Incorrect: multiple myeloma is associated with deletions in the long arm of chromosome 13 and a variety of chromosomal rearrangements affecting the IgH gene.


Citation: Kumar: Robbins & Cotran Pathologic Basis of Disease, Eighth Edition, 2009

Source: BoardVitals, 2/5/2014