Stains & molecular markers
Cyclin D1 / BCL1
Copyright: 2003-2021, PathologyOutlines.com, Inc.
PubMed Search: Cyclin+D1[title]
Cyclin D1 / BCL1
Author: Nat Pernick, M.D.
Topic Completed: 1 March 2013
Minor changes: 9 December 2020
Copyright: 2003-2021, PathologyOutlines.com, Inc.
PubMed Search: Cyclin+D1[title]
Table of Contents
Definition / general | Uses by pathologists | Microscopic (histologic) images | Positive staining - normal | Positive staining - disease | Negative staining | Board review style question #1 | Board review style answer #1Cite this page: Pernick, N. Cyclin D1 . PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/stainscyclind1.html. Accessed January 20th, 2021.
Definition / general
- Cyclin D1 is also known as BCL1, B Cell Lymphoma #1, CCND1
- Coded by BCL1 / PRAD1 (parathyroid adenoma 1) gene on #11q13
- Responsible for transition to S phase by phosphorylating the retinoblastoma gene product, which releases transcription factors to initiate DNA replication
- Overexpression promotes transformation to a malignant phenotype; overexpressed in many tumors
- Cyclin D1 genotypes may be associated with nasopharyngeal carcinoma risk (Anticancer Res 2012;32:1093)
Uses by pathologists
- Interpretation: nuclear stain
- Mantle cell lymphoma: characterized by t(11;14) translocation, which places the cyclin D1 gene next to an immunoglobulin heavy chain enhancer gene; among lymphomas, cyclin D1 expression is very specific for mantle cell lymphoma; however, blastoid cases may be negative (Am J Surg Pathol 2012;36:214)
- Parathyroid: overexpressed in 40% of parathyroid adenomas, strong staining in carcinomas
Microscopic (histologic) images
Case #260
Breast: MALT
lymphoma is
cyclin D1 negative
Images hosted on other servers:
Breast: papilloma and papillary carcinoma
Cervix: various benign and malignant lesions
Mantle cell lymphoma (small bowel)
Mantle cell lympoma (axillary lymph node)
Skin: normal
Stomach: gastric or
gastroesophageal
junction
adenocarcinoma
Various images
Positive staining - normal
- Endocervix (Mod Pathol 2010;23:611)
- Uterus: cycling endometrium
Positive staining - disease
-
Positive staining is nuclear staining:
- B-CLL (13%, Am J Surg Pathol 2004;28:801)
- Diffuse large B cell lymphoma (rare, Hum Pathol 2011;42:1204)
- Soft tissue: chondroid lipoma, Gardner type fibroma
- Hairy cell leukemia (25%)
- Inflammatory fibroid polyp
- Liver: Hepatoblastoma (some)
- Mantle cell lymphoma
- Myeloma (40%)
- Pancreas: solid pseudopapillary neoplasm
- Parathyroid adenoma (40%) and carcinoma (90%)
- Salivary gland: adenoid cystic carcinoma
- Splenic lymphoma with villous lymphocytes (10%)
- Uterus: endometrial stromal sarcoma-undifferentiated (Mod Pathol 2010;23:225, Am J Surg Pathol 2012;36:1562)
- Various carcinomas (Hum Pathol 2012;43:1386, Hum Pathol 2012;43:1044) and melanoma
Negative staining
Board review style question #1
A t(11;14) translocation that creates a CyclinD1-IgH fusion gene is associated with which of the malignancies below?
- Burkitt lymphoma
- Extranodal marginal zone lymphoma
- Follicular lymphoma
- Mantle cell lymphoma
- SLL / CLL
Board review style answer #1
D. Mantle cell lymphoma.
Citation: Kumar: Robbins & Cotran Pathologic Basis of Disease, Eighth Edition, 2009
Source: BoardVitals, 2/5/2014
- Incorrect: Burkitt lymphomas are associated with translocations involving the c-MYC gene on chromosome 8.
- Incorrect: extranodal marginal zone lymphomas are associated with the following translocations: t(11;18) (creating a MALT1-IAP2 fusion gene), t(1;14)(creating a BCL10-IgH fusion gene) and t(14;18) (creating a MALT1-IgH fusion gene).
- Incorrect: follicular lymphoma is associated with a t(14;18) translocation that creates a BCL2-IgH fusion gene.
- Incorrect: SLL / CLL is associated with trisomy 12, deletion 13q14 and deletion 11q23.
Citation: Kumar: Robbins & Cotran Pathologic Basis of Disease, Eighth Edition, 2009
Source: BoardVitals, 2/5/2014
