Stains & CD markers
Cyclin D1 / BCL1


Topic Completed: 24 August 2021

Minor changes: 24 August 2021

Copyright: 2003-2021, PathologyOutlines.com, Inc.

PubMed Search: Cyclin D1[TI] pathology[TIAB]

Qurratulain Chundriger, M.B.B.S.
Zubair Ahmad, M.B.B.S.
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Cite this page: Chundriger Q, Rahim S, Ahmad Z. Cyclin D1. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/stainscyclind1.html. Accessed October 24th, 2021.
Definition / general
  • Member of cyclin family of cell cycle regulators; 11q13.3 locus
Essential features
  • Cell cycle regulator that controls transition from G1 to S phase in normal tissues
  • Mutation leads to increased cell proliferation via disruption of downstream pathways, resulting in tumorigenesis
  • Expression by immunohistochemistry is valuable in diagnosis of some tumors as well as predicting prognosis in a number of tumors
Terminology
  • BCL1 (B cell lymphoma 1)
  • CCND1
  • PRAD1 (parathyroid adenomatosis 1)
Pathophysiology
  • Cyclin D1 is activated in the G1 phase of the cell cycle; it activates CDK4, followed by activation of cyclin / CDK (cyclin dependent kinase) complexes leading to progression of the cell cycle to S phase (Proc Natl Acad Sci U S A 2020;117:17177)
  • Also regulates transcription in different cell models (J Clin Invest 2018;128:4132)
  • Tumorigenesis: it binds to either CDK4 or CDK6, resulting in phosphorylation of pRB, E2F release and subsequent promotion of the G1 / S phase transition (Nat Rev Cancer 2007;7:750)
Clinical features
Interpretation
  • Nuclear staining
Uses by pathologists
Prognostic factors
Case reports
Microscopic (histologic) description
  • Staining is predominantly nuclear
Microscopic (histologic) images

Contributed by Nasir Ud Din, M.B.B.S.
Ewing sarcoma

Ewing sarcoma

Ewing sarcoma cyclin D1

Ewing sarcoma cyclin D1

Endometrial stromal sarcoma

Endometrial stromal sarcoma

Endometrial stromal sarcoma cyclin D1

Endometrial stromal sarcoma cyclin D1

Wilms tumor

Wilms tumor


Wilms tumor cyclin D1

Wilms tumor cyclin D1

Mantle cell lymphoma

Mantle cell lymphoma

Mantle cell lymphoma cyclin D1

Mantle cell lymphoma cyclin D1

Solid pseudopapillary neoplasm

Solid pseudopapillary neoplasm

Solid pseudopapillary neoplasm cyclin D1

Solid pseudopapillary neoplasm cyclin D1

Positive staining - normal
Positive staining - disease
Negative staining
Sample pathology report
  • Cervical lymph node, excision biopsy:
    • Mantle cell lymphoma (see comment)
    • Comment: The tumor expresses CD5 and cyclin D1 in addition to other B cell markers, with a low proliferative index and negative CD23, consistent with a diagnosis of mantle cell lymphoma. Further molecular testing and clinical workup is recommended.
Board review style question #1
Figure A

Figure A

Figure B

Figure B



A 54 year old man presents with low grade fever, malaise and enlarged cervical and supraclavicular lymph nodes. The biopsy from one of the cervical nodes shows effaced architecture and sheet-like growth of small sized lymphocytes with irregular nuclear contours (Figure A). Immunohistochemical analysis shows positivity for pan B cell markers, CD5 and cyclin D1 (Figure B). The diagnosis in this case is

  1. Follicular lymphoma
  2. Hairy cell leukemia
  3. Mantle cell lymphoma
  4. Marginal zone lymphoma
  5. Small lymphocytic lymphoma
Board review style answer #1
C. Mantle cell lymphoma. Follicular lymphoma (answer A) is negative for CD5 and shows positivity for CD10. Hairy cell leukemia (answer B) may show positivity for cyclin D1 but is negative for CD5 and shows positivity for annexin A and other stains. Marginal zone lymphoma (answer D) is negative for both CD5 and cyclin D1. Small lymphocytic lymphoma (answer E) is the main differential diagnosis for CD5 positive B cell lymphomas and may show dim cyclin D1 positivity in proliferation centers.

Comment Here

Reference: Cyclin D1
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