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Stains
Immunohistochemistry (IHC) procedure

Reviewer: Nat Pernick, M.D. (see Reviewers page)
Revised: 29 July 2013, last major update July 2013
Copyright: (c) 2002-2013, PathologyOutlines.com, Inc.

General
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Alternative methods
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Diagrams
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Direct method (uses only one antibody)


Indirect method (uses primary and secondary antibodiesy)

Uses by pathologists
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Micro images
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Membranous staining pattern:
stain localizes along cell membrane


Cytoplasmic staining pattern (left-inhibin, right-beta hCG): stain is diffuse within cytoplasm


Dot-like staining pattern

Golgi staining pattern: prolactin


Nuclear staining pattern: ER in invasive ductal carcinoma

Nuclear staining pattern: p63 in myoepithelial carcinoma

Videos
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End of Stains > Immunohistochemistry (IHC) procedure


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