Stains
p63

Author: Nat Pernick, M.D. (see Authors page)

Revised: 15 November 2016, last major update April 2012

Copyright: (c) 2002-2015, PathologyOutlines.com, Inc.

PubMed Search: p63 [title]

Cite this page: p63. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/stainsp63.html. Accessed December 10th, 2016.
Definition / General
  • Member of p53 gene family at 3q27-29, but does not appear to be a tumor suppressor gene
  • Myoepithelial marker; others are smooth muscle myosin heavy chains, calponin, p75, P-cadherin, basal cytokeratins (CK 5 / 6), maspin, and CD10 (Arch Pathol Lab Med 2011;135:422)
Pathophysiology
  • Encodes at least 6 different proteins with different biologic functions
  • Appears to regulate growth and development of epithelial organs (J Biomed Biotechnol 2011;2011:864904), as germline mutations cause ectrodactyly (missing or irregular fingers or toes), ectodermal dysplasia and facial clefts syndrome, also called limb mammary syndrome (hypoplasia / aplasia of mammary glands and nipple)
  • May be molecular switch for initiation of "epithelial stratification program"
  • Regulates human keratinocyte proliferation (J Biol Chem 2012;287:5627), olfactory stem cell self renewal and differentiation (Neuron 2011;72:748)
  • Gene and protein expression may not correlate due to presence of isoforms and post translation modification
  • Main isoforms are TAp63 and deltaNp63
  • TAp63 ("full length" p63) activates p53 target genes, but deltaNp63 inhibits transcription activation of p53 gene and transactivating isoforms
  • TAp63 is highly expressed in most benign tumors; negative / weak in most carcinomas, but deltaNp63 is negative / weak in most benign tumors and highly expressed in adenoid cystic, mucoepidermoid and myoepithelial carcinomas (Hum Pathol 2005;36:821)
Interpretation
  • Typically a nuclear stain
  • Cytoplasmic staining of Z-bands of benign and malignant skeletal muscle tumors (Mod Pathol 2011;24:1320)
Uses by Pathologists
Micro Images
Scroll to see all images.

Images hosted on PathOut server:

Thyroid, Courtesy of Andrey Bychkov, M.D., Ph.D.

p63+ cells found in many follicles

Intense immunostaining
correlates with a loss
of TTF-1 expression



Breast:

Missing Image

Adenoid cystic carc, solid



Nasal septum:

Missing Image Missing Image

Seromucinous hamartoma p63−, no myoepithelial cells, positive internal control



Prostate:

Missing Image

Nephrogenic adenoma

Missing Image Missing Image

Pseudohyperplastic adenocarcinoma



Images hosted on other servers:

Anus:

Missing Image Missing Image

Normal and carcinoma anal glands



Bladder / Other GU:

Missing Image

Normal and carcinoma urothelium

Missing Image Missing Image

Invasive UC



Bone:

Missing Image Missing Image

Various bone tumors



Breast:

Missing Image

Papilloma

Missing Image

Tubular adenoma

Missing Image Missing Image

Nipple adenoma, p63 shows intact myoepithelial layer

Missing Image

p63, Fig.C

Missing Image

Medullary carcinoma

Missing Image

Myoepithelial carcinoma



Lymph nodes:

Missing Image

Benign epithelial inclusions



Lymphoma:

Missing Image

Anaplastic large cell lymphoma

Missing Image

Hodgkin lymphoma



Maxilla:

Missing Image

Nasolabial cyst

Missing Image

Respiratory epithelial adenomatoid hamartoma



Nasal septum:

Missing Image

Intraductal carcinoma



Skin:

Missing Image

Syringocystadeno
carcinoma papilliferum

Missing Image

Sweat gland carcinoma



Uterus:

Missing Image

Endometriosis

Positive Staining - Normal
  • Breast: myoepithelium (Am J Surg Pathol 2001;25:1054), Toker cells
  • Gynecologic tract: basal and parabasal cells of mature cervical, vaginal and vulval squamous epithelium; cervical reserve cells at transformation zone, immature metaplastic and atrophic cervical squamous epithelium (Adv Anat Pathol 20 09;16:316)
  • Lung: bronchial reserve cells; metaplastic squamous bronchial epithelium (lower strata)
  • Also prostate (basal cells), skin (basal cells), thymus (epithelial cells), urothelium
Positive Staining - Disease
Negative Staining