Stains & molecular markers
BerEP4 / EpCAM


Topic Completed: 1 August 2015

Minor changes: 2 September 2020

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PubMed Search: BerEP4 / EpCAM

Nat Pernick, M.D.
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Cite this page: Pernick N. BerEP4 / EpCAM. PathologyOutlines.com website. https://www.pathologyoutlines.com/topic/stainsepcam.html. Accessed October 26th, 2020.
Definition / general
Uses by pathologists
  • Membranous staining
  • Sensitive and specific for lung adenocarcinoma (positive) vs. mesothelioma (negative, Am J Surg Pathol 2001;25:43)
  • May help distinguish, as part of a panel, hepatocellular carcinoma (usually negative) from metastatic adenocarcinoma to liver or cholangiocarcinoma (usually positive, Mod Pathol 2002;15:1279)
  • Immunoexpression may predict poor survival in carcinomas of breast, gallbladder (Am J Clin Pathol 2008;129:424), ovary, pancreas
Microscopic (histologic) images

Contributed by Jijgee Munkhdelger, M.D., Ph.D. and Andrey Bychkov, M.D., Ph.D.

Ovarian serous carcinoma immunoprofile



Images hosted on other servers:

Basaloid squamous cell carcinoma is BerEP4+

Breast: ductal and
lobular carcinoma -
primary and metastases
(EpCAM)

Breast cancer: increased EpCAM expression in metastases


Colon: loss of EPCAM expression in cancers associated with Lynch syndrome caused by heterozygous EPCAM germline deletions

Esophagus: normal
and Barrett metaplasia;
gastric mucosa

Unknown site: adenocarcinoma (MOC31)

Cytology images

Images hosted on other servers:

Reactive mesothelial cells (BerEP4 / MOC31 negative) versus metastatic adenocarcinoma (positive)

Positive staining - normal
  • Basolateral surface of epithelial cells
Positive staining - disease
Negative staining
  • Epidermal keratinocytes, gastric parietal cells, hepatocytes, myoepithelial cells, squamous epithelia, thymic cortical epithelium (Front Biosci 2008;13:3096)
  • Lymphoma, mesothelioma, most soft tissue sarcomas
  • Adenomatoid tumor; renal oncocytoma
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