BerEP4 / EpCAM

Topic Completed: 1 August 2015

Minor changes: 4 March 2019

Copyright: 2003-2018,, Inc.

PubMed Search: BerEP4 / EpCAM

Nat Pernick, M.D.
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Page views in 2020 to date: 13,805
Cite this page: Pernick N. BerEP4 / EpCAM. website. Accessed July 13th, 2020.
Definition / general
Uses by pathologists
  • Membranous staining
  • Sensitive and specific for lung adenocarcinoma (positive) vs. mesothelioma (negative, Am J Surg Pathol 2001;25:43)
  • May help distinguish, as part of a panel, hepatocellular carcinoma (usually negative) from metastatic adenocarcinoma to liver or cholangiocarcinoma (usually positive, Mod Pathol 2002;15:1279)
  • Immunoexpression may predict poor survival in carcinomas of breast, gallbladder (Am J Clin Pathol 2008;129:424), ovary, pancreas
Microscopic (histologic) images

Images hosted on other servers:

Basaloid squamous cell carcinoma is BerEP4+

Breast: ductal and
lobular carcinoma -
primary and metastases

Breast cancer: increased EpCAM expression in metastases

Colon: loss of EPCAM expression in cancers associated with Lynch syndrome caused by heterozygous EPCAM germline deletions

Esophagus: normal
and Barrett metaplasia;
gastric mucosa


Fig E: Ovarian serous
carcinoma is MOC31+
Fig F: lung adenocarcinoma
is BerEP4+


Unknown site: adenocarcinoma (MOC31)

Cytology images

Images hosted on other servers:

Reactive mesothelial cells (BerEP4 / MOC31 negative) versus metastatic adenocarcinoma (positive)

Positive staining - normal
  • Basolateral surface of epithelial cells
Positive staining - disease
Negative staining
  • Epidermal keratinocytes, gastric parietal cells, hepatocytes, myoepithelial cells, squamous epithelia, thymic cortical epithelium (Front Biosci 2008;13:3096)
  • Lymphoma, mesothelioma, most soft tissue sarcomas
  • Adenomatoid tumor; renal oncocytoma
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