Vulva, vagina & female urethra
Vulva - carcinoma and precursor lesions
Differentiated type, vulvar intraepithelial neoplasia (dVIN)


Topic Completed: 12 May 2020

Minor changes: 12 May 2020

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PubMed Search: dVIN

Matthias Choschzick, M.D.
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Cite this page: Choschzick M. Differentiated type, vulvar intraepithelial neoplasia. PathologyOutlines.com website. http://www.pathologyoutlines.com/topic/vulvadifferentiatedVIN.html. Accessed May 30th, 2020.
Definition / general
  • Differentiated vulvar intraepithelial neoplasia (dVIN) is the putative precursor lesion of HPV independent vulvar squamous cell carcinoma
Essential features
  • Highly differentiated with histological overlapping features to reactive or benign vulvar lesions
  • Currently, there are no reliable immunohistochemical or molecular markers to establish the diagnosis
  • Differentiated vulvar intraepithelial neoplasia is an aggressive lesion with higher potential to become invasive than HPV associated usual type vulvar intraepithelial neoplasia
Terminology
  • Old: simplex vulvar intraepithelial neoplasia
ICD coding
  • ICD-10: D07.1 - carcinoma in situ of vulva
  • ICD-O: 8071/2 - differentiated type vulvar intraepithelial neoplasia
Epidemiology
  • Postmenopausal women, sixth to eighth decade, 2 - 29% of all vulvar intraepithelial neoplasia types (Pathology 2016;48:291)
Sites
  • Labia minora, commonly found adjacent to invasive squamous cell carcinoma (70%)
Etiology
Clinical features
  • Unifocal gray-white papules or plaques
Diagnosis
  • Difficult to diagnose in punch biopsies alone; overlapping histological features with other common vulvar lesions, especially lichen simplex and others
Prognostic factors
Case reports
Treatment
Microscopic (histologic) description
  • Acanthosis, parakeratosis, elongation and anastomosis of rete ridges, keratin pearls (Histopathology 2020;76:128)
  • Mild to moderate atypical cells in basal and parabasal layers with hyperchromatic nuclei, lacks full thickness atypia
  • Enlarged squamous cells with eosinophilic cytoplasm, vesicular nuclei and prominent nucleoli (premature differentiation), prominent intercellular bridges
  • Rare basaloid differentiation with atypical cells in upper cell layers (Am J Surg Pathol 2009;33:1659)
Microscopic (histologic) images

Contributed by Matthias Choschzick, M.D.

Acanthosis, rete elongation and atypia

p53

Positive stains
Negative stains
Molecular / cytogenetics description
Sample pathology report
  • Labium minus, biopsy:
    • Differentiated vulvar intraepithelial neoplasia (see comment)
    • Comment: p16 is negative and p53 is overexpressed in an aberrant pattern.
Differential diagnosis
Board review style question #1


The picture above shows an example of HPV negative squamous cell carcinoma of the vulva in association to differentiated vulvar intraepithelial neoplasia. Which gene is most relevant for the pathogenesis of this subgroup of vulvar cancer?

  1. BRCA1
  2. CDKN2A
  3. HRAS
  4. PIK3CA
  5. TP53
Board review style question #2
Which immunohistochemical stain shows an aberrant (mutation type) pattern in in differentiated vulvar intraepithelial neoplasia?

  1. CK17
  2. Ki67
  3. p16
  4. p53
  5. SOX2
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